Project

Part:BBa_K2805013:Design

Designed by: Cassandra Scott Leach   Group: iGEM18_BostonU   (2018-10-10)


LOV2 -> vioC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1457
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1086
    Illegal EcoRI site found at 1457
    Illegal BglII site found at 369
    Illegal BglII site found at 1826
    Illegal BamHI site found at 126
    Illegal BamHI site found at 1583
    Illegal BamHI site found at 3128
    Illegal XhoI site found at 3137
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1457
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1457
    Illegal NgoMIV site found at 1406
    Illegal NgoMIV site found at 3448
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

oof


Source

UW codon optimized vioC in 2017

References