Project
Part:BBa_K2805013:Design
Designed by: Cassandra Scott Leach Group: iGEM18_BostonU (2018-10-10)
LOV2 -> vioC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1457
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1457
- 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 1086
Illegal EcoRI site found at 1457
Illegal BglII site found at 369
Illegal BglII site found at 1826
Illegal BamHI site found at 126
Illegal BamHI site found at 1583
Illegal BamHI site found at 3128
Illegal XhoI site found at 3137 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1457
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1457
Illegal NgoMIV site found at 1406
Illegal NgoMIV site found at 3448 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
oof
Source
UW codon optimized vioC in 2017